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pan-ifn- α enzyme-linked immunosorbent assay (elisa) kit  (Mabtech Inc)

 
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    Mabtech Inc pan-ifn- α enzyme-linked immunosorbent assay (elisa) kit
    Pan Ifn α Enzyme Linked Immunosorbent Assay (Elisa) Kit, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pan-ifn- α enzyme-linked immunosorbent assay (elisa) kit/product/Mabtech Inc
    Average 90 stars, based on 1 article reviews
    pan-ifn- α enzyme-linked immunosorbent assay (elisa) kit - by Bioz Stars, 2026-03
    90/100 stars

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    EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

    Journal: Blood Advances

    Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

    doi: 10.1182/bloodadvances.2018025536

    Figure Lengend Snippet: EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

    Article Snippet: IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

    Journal: Blood Advances

    Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

    doi: 10.1182/bloodadvances.2018025536

    Figure Lengend Snippet: EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

    Article Snippet: IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

    EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

    Journal: Blood Advances

    Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

    doi: 10.1182/bloodadvances.2018025536

    Figure Lengend Snippet: EBV infection elicits type I IFN production in PBMCs in a pDC-dependent manner. Total PBMCs were exposed to EBV, CpG C, Poly I:C, or R848 at the indicated concentrations, and culture supernatants were collected after 18 to 24 hours and analyzed for cytokine production according to enzyme-linked immunosorbent assay. IFN-α (A) and IL-12p40 (B) content was measured. (C) PBMCs were inoculated with wild-type EBV (wt), EBNA3A-deficient EBV (3aKO), or EBNA3C-deficient EBV (3cKO), and IFN-α production was measured. MOI 0.005, n = 5; MOI 0.05, n = 7; and MOI 0.25, n = 9. (D) Total PBMCs were compared with pDC-depleted PBMCs. MOI 0.005, n = 2; MOI 0.05, n = 4; and MOI 0.25, n = 2. Paired Student t tests were used for all graphs.

    Article Snippet: 27 , 29 , 31 Enzyme-linked immunosorbent assay IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

    Journal: Blood Advances

    Article Title: Plasmacytoid dendritic cells respond to Epstein-Barr virus infection with a distinct type I interferon subtype profile

    doi: 10.1182/bloodadvances.2018025536

    Figure Lengend Snippet: EBV infection elicits type I IFN production in huNSG mice. huNSG mice were infected with 5 × 105 RGU EBV, and blood samples were analyzed 18 hours postinfection. IFN-α (A) or IL-12p40 (B) production was measured in the serum by enzyme-linked immunosorbent assay (PBS, n = 11; EBV, n = 12). (C) IFN-α levels were compared in the serum of huNSG mice that were PBS treated (n = 6) or infected with either 5 × 105 RGU of wild-type EBV (wt, n = 6), EBNA3A-deficient EBV (3aKO, n = 6), or EBNA3C-deficient EBV (3cKO, n = 8). (D) ISG induction was measured in blood leukocyte lysates by using quantitative real-time polymerase chain reaction. n = 8 for PBS; n = 9 for EBV. Unpaired Student t tests were applied.

    Article Snippet: 27 , 29 , 31 Enzyme-linked immunosorbent assay IFN-α Pan and IL-12p40 enzyme-linked immunosorbent assay kits were purchased from Mabtech AB, and assays were performed according to the manufacturer’s recommendations.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction